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Improve post-thaw cell recovery by up to 80%

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INTRODUCTION

CytoBoost Revive is a cell culture additive that dramatically improves survival of iPSCs, organoids, and sensitive cell lines after cryopreservation. Recovering up to 4× more viable cells after thawing.

Designed for stem cells, organoids, and primary cultures, it helps your cells recover faster, reducing failed experiments, saving time, and protecting valuable samples. It will dramatically improve survival, attachment and functional recovery of sensitive cells.

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THE PROBLEM

Why Cryopreservation Damages Cells

  Membrane damage

  Oxidative stress

  Cytoskeletal disruption

  Loss of attachment capability

  Apoptosis activation

This often results in:

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  Low viability

  Poor attachment

  Delayed recovery

  Failed cultures

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Cryopreservation places extreme stress on cells.

During freeze-thaw, cells experience:

For sensitive cells such as iPSCs and organoids, recovery rates can drop below 20%.

THE SOLUTION

How CytoBoost Revive Improves Cell Survival

CytoBoost Revive supports cells during the critical post-thaw recovery window.

It works by helping cells:

✓  Stabilise cellular structures

✓  Improve attachment efficiency

✓  Reduce post-thaw stress

✓  Accelerate recovery and growth

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This leads to lost time, wasted resources, and failed experiments.

PROVEN RESULTS

Recovering up to 4× more viable cells

CytoBoost Revive has demonstrated significant improvements in recovery across multiple cell types.

Typical results include:

✓  Increase recovery from ~20% to over 80%

✓  Faster attachment

✓  Improved morphology

✓  More consistent culture establishment

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Particularly effective for:

✓  Induced pluripotent stem cells (iPSCs)

✓  Organoids

✓  Primary cells

✓  Sensitive cell lines

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KEY BENEFITS

✓ Improve viability - recover more usable cells after thawing

✓ Save time and reduce failed experiments - avoid repeating cryopreservation and recovery cycles

✓ Simple integration - add directly to your existing culture medium

✓ Compatible with sensitive cells - optimised for stem cells and organoids

✓ Consistent results - improve reproducibility across experiments

✓ Reduce culture variability - support more uniform recovery, improving experimental reliability and data quality

How CytoBoost Revive can help you:

APPLICATIONS

CytoBoost Revive is ideal for:

✓ iPSC culture recovery - improve post-thaw survival of pluripotent stem cells 

✓ Organoid thaw and recovery - enhance recovery of complex 3D organoids

✓ Stem cell research - support reliable recovery of pluripotent and multipotent stem cells

✓ Primary cell recovery - increase viability/attachment of fragile primary cells

✓ Cryopreserved cell banks - maximise recovery when restarting cultures 

✓ Drug discovery workflows - ensure reproducible recovery of assay-ready cells

✓ Regenerative medicine research - support recovery in advanced therapeutic and translational applications

FAQs

What is CytoBoost™ REVIVE

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TESTIMONIALS

"I would highly recommend CytoBoost™. Its ability to reduce the need for an active agent while improving results makes it a cost-effective solution." 

PhD Student. Maria Lazari

Newcastle University

DOWNLOADS

Information Sheet

Reduces post-thaw shock and CIDOCD

Real Life Application

Improved iPSC recovery

from 20% to over 80%

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