


Improve post-thaw cell recovery by up to 80%

INTRODUCTION
CytoBoost Revive is a cell culture additive that dramatically improves survival of iPSCs, organoids, and sensitive cell lines after cryopreservation. Recovering up to 4× more viable cells after thawing.
Designed for stem cells, organoids, and primary cultures, it helps your cells recover faster, reducing failed experiments, saving time, and protecting valuable samples. It will dramatically improve survival, attachment and functional recovery of sensitive cells.


THE PROBLEM
Why Cryopreservation Damages Cells
⚠ Membrane damage
⚠ Oxidative stress
⚠ Cytoskeletal disruption
⚠ Loss of attachment capability
⚠ Apoptosis activation
This often results in:
⚠ Low viability
⚠ Poor attachment
⚠ Delayed recovery
⚠ Failed cultures

Cryopreservation places extreme stress on cells.
During freeze-thaw, cells experience:
For sensitive cells such as iPSCs and organoids, recovery rates can drop below 20%.
THE SOLUTION
How CytoBoost Revive Improves Cell Survival
CytoBoost Revive supports cells during the critical post-thaw recovery window.
It works by helping cells:
✓ Stabilise cellular structures
✓ Improve attachment efficiency
✓ Reduce post-thaw stress
✓ Accelerate recovery and growth

This leads to lost time, wasted resources, and failed experiments.
PROVEN RESULTS
Recovering up to 4× more viable cells
CytoBoost Revive has demonstrated significant improvements in recovery across multiple cell types.
Typical results include:
✓ Increase recovery from ~20% to over 80%
✓ Faster attachment
✓ Improved morphology
✓ More consistent culture establishment

Particularly effective for:
✓ Induced pluripotent stem cells (iPSCs)
✓ Organoids
✓ Primary cells
✓ Sensitive cell lines

KEY BENEFITS
✓ Improve viability - recover more usable cells after thawing
✓ Save time and reduce failed experiments - avoid repeating cryopreservation and recovery cycles
✓ Simple integration - add directly to your existing culture medium
✓ Compatible with sensitive cells - optimised for stem cells and organoids
✓ Consistent results - improve reproducibility across experiments
✓ Reduce culture variability - support more uniform recovery, improving experimental reliability and data quality
How CytoBoost Revive can help you:
APPLICATIONS
CytoBoost Revive is ideal for:
✓ iPSC culture recovery - improve post-thaw survival of pluripotent stem cells
✓ Organoid thaw and recovery - enhance recovery of complex 3D organoids
✓ Stem cell research - support reliable recovery of pluripotent and multipotent stem cells
✓ Primary cell recovery - increase viability/attachment of fragile primary cells
✓ Cryopreserved cell banks - maximise recovery when restarting cultures
✓ Drug discovery workflows - ensure reproducible recovery of assay-ready cells
✓ Regenerative medicine research - support recovery in advanced therapeutic and translational applications
FAQs
What is CytoBoost™ REVIVE






